Blood And Tissues

TITLE: BLOOD & TISSUES- Staining Of Blood Films & Identification Of Leukocytes. OBJECTIVES: i.To numerate the preparation of derivation films from human blood sample. ii.To learn the techniques of tarnish the blood films with Giemsa filthiness and Wright territory. iii.To identify and make drawing of leukocytes that gratuity in human blood sample under microscope. interpellation: Leukocytes argon divided into granulocytes and agranulocytes. Granulocytes ar also known as polymorphonuclear leukocytes, or polys for short. Three types of granulocyte are neutrophils, bromeosinophils, basophils. Meanwhile, there are ii types of agranulocyctes which are lymphocytes and monocytes. If we observe the smear as it is after fixing, we guide on on befool really little because all cells are very transparent. The erythrocytes are slightly visible, but the leukocytes are in any case pale, just about invisible and we will not see anything inside them. To be able to obse rve and recognize the dissimilar kinds of leukocyte, we must dirt them. For this purpose, normally Giemsa dent and Wright stain are used. Giemsa stain is a mixture of dyes, based on methylene group blue (basic dye) and eosin ( acrid dye). Giemsa stain is a classical blood film stain for peripheral blood smears and bone spunk specimens.

The principle of Giemsa stain is the basic dye stain the acidic sectionalisation of the burden of the cell and the acidic dye stained the basic part of the cytoplasm of the cell. Therefore, the Giemsa stain allows clear observations to oppose types of leukocytes. By patch of the cells we can observe the different type! s of ashen blood cells (leukocyctes) which are, neutrophils, eosinophils, basophils, lymphocytes and monocytes under the light microscope. MATERIALS: 1)Alcohol swabs 2)Blood Lancets 3)Deionized water 4)Distilled water 5)Giemsa stain 6)Glass Slides 7)Jar of disinfection for disposal of slides 8)Methanol 9)Microscopes 10)Oil Immersion 11)Pasteur pipettes 12)Sorensens...If you want to get a expert essay, fix it on our website: OrderCustomPaper.com

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