The Dna Extraction Essay Example for Free

The Dna Extraction Essay1. The salt contri thates positively charged atoms that neutralise the common negative charge of desoxyribonucleic acid. Salt is used at a high molarity due to the accompaniment that it precipitates building block of the proteins out. deoxyribonucleic acid is insoluble in low molar salt replys but soluble in low molar salt radicals thus keeping the deoxyribonucleic acid in solvent. 2. blending the onion result homogenize the garland and it helps with the kick in trim of the cell w anys. Blending saves wizard the time and effort of using a motar and pestle, however it may break a lot of the DNA which is non favourable as one needs a lot of DNA for the extraction. 3. The enzymes in the soap are used to break down the lipid (fat) molecules of the cells nuclear membranes releasing the contents of the cell crucially including the DNA. These enzymes in the soap are what break down grease while washing dishes.4. The DNA does not dissolve in this a lcohol but rather pushes up through and out of the solution or precipitates. It is less dense than peeing or cell scum which is what settles to the croup of the glass so it floats up into the alcohol layer, where you see it as a snotty, string-like substance, with small bubbles formed on it. 5. Because protein is stored in them for the junkyrition of the new plants. 6. It is too small to be seen with the defenseless eye. What you extracted is millions of strands of DNA. In addition to that, whilst the substance was heated, the DNA got denatured which results in it looking more like a ladder than a helix. 7. Most of the DNA extracted during this proccess comes from the nucleus of the cell.AIMThe purpose of this experiment is to extract DNA from a variety of cells (Onion cells in particular) and see DNA molecules. This will show that, contrary to popular opinion, DNA is not just found in blood cells, but in a variety of tissues. preliminary knowledge should include the fact that cell membranes are layers of lipids, or fat molecules, that DNA is found in the nucleus of a cell, and that enzymes speed up chemical reactions. HYPOTHESIS DNA is present in the cells of all living organisms.METHOD1. Prepare two water bathrooms one at 60C and some other filled with ice and water, around 4C. For the hot water bath, a large metal fate piece of tail be used along with a thermometer with an appropriate temperature range. For the ice bath, a mixing paradiddle filled with ice and water works well. 2. For each onion, make a solution consisting of 10 ml of liquid dishwashing detergent and 1.5 g of table salt. Put in a 250 ml beaker and emulsify. 3. check distilled water to make a final volume of 100 ml. Dissolve the salt by divine guidance slowly to avoid foaming. 4. Coarsely chop one large onion with a food central processor or blender and put into a 1000 ml mixing bowl. For best results, do not chop the onion too finely. The size of the pieces should be like thos e used in making spaghetti. It is stop to have the pieces too large than too small.5. Cover chopped onion with the 100 ml of solution from rate 2. The liquid detergent causes the cell membrane to break down and dissolves the lipids and proteins of the cell by disrupting the bonds that render the cell membrane together. The detergent causes lipids and proteins to precipitate out of the solution. Salt enables nucleic acids to precipitate out of an alcohol solution because it shields the negative phosphate end of DNA, causing the DNA strands to come closer together and coalesce. 6. Put the cadence cup in a hot water bath at 60C for 10-12 minutes. During this time, inspire the chopped onion mixture against the side of the measuring cup with the back of the spoon. (Do not keep the mixture in the hot water bath for more than 15 minutes because the DNA will come out to break down.) If using a large metal pot for water bath, remove the pot from the fit out before placing the onion-co ntaining measuring cup insidethe procedure is safer if the pot is off the burner. Continue to monitor temperature of water bath and make adjustments as needed.7. The heat treatment softens the phospholipids in the cell membrane and denatures the DNAse enzymes which, if present, would cut the DNA into small fragments so that it could not be extracted. 8. Cool the mixture in an ice water bath for 5 minutes. During this time, press the chopped onion mixture against the side of the measuring cup with the back of the spoon. This step slows the breakdown of DNA. 9. Filter the mixture through a 6 coffee filter or quadruple layers of cheese cloth placed in a strainer over a 4-cup measuring cup. When you filter the onion mixture, try to keep the foam from getting into the filtrate. It sometimes filters slowly, so you might want to put the whole set up in the refrigerator and let it filter overnight. 10. Dispense the onion solution into a rise tubing. The test tube should contain about 1 t easpoon of solution or be about 1/3 full. For most uniform results among test tubes, stir the solution frequently when dispensing it into the tubes. at that place is not an advantage to dispensing more than one teaspoon of solution into a test tube. The solution can be stored in a refrigerator for about a day before it is used for the laboratory exercise. When the solution is removed from the refrigerator, it should be gently mixed before the test tubes are filled.11. Add heatless alcohol to the test tube to create an alcohol layer on top of about 1 cm. For best results, the alcohol should be as cold as possible. The alcohol can be added to the solution in at least three ways (a) Fill a pasteur pipette with alcohol, put it to bottom of the test tube, and release the alcohol. (b) Or, put about 1 cm of alcohol into the bottom of a test tube and add the onion solution. (c) Or, slowly pour the alcohol down the inside of the test tube with a pasteur pipette or medicine dropper. DNA i s not soluble in alcohol. When alcohol is added to the mixture, all the components of the mixture, except for DNA, stay in solution while the DNA precipitates out into the alcohol layer. 12. let the solution sit for 2-3 minutes without disturbing it. It is important NOT to shake the test tube. You can ensure the white DNA precipitate out into the alcohol layer. When good results are obtained, there will be enough DNA to spool on to a glass rod, a pasteur pipette that has been heated at the tip to form a hook, or similar device. A wooden skewer or nut pick (small metal rod with curved tip) may also work well for spooling DNA if Pasteur pipette is unavailable. DNA has the appearance of white mucus.CONCLUSIONWith these findings we can conclude that DNA is present and can be found in the cells of all living organisms and not just in those of the human body.